Peptide Linker Designer

Amino Acid Sequence

Deuteration Level (%)

0% 50% 100%

Enzyme Selection

Cathepsin B Neutrophil Elastase

Predicted Cleavage Sites

Results

Position	Enzyme	Cleavage Probability	Recommendation
1-2	Cathepsin B	High	Deuterate position 2

Enzyme Activity Comparison

Deuteration Pattern

Cleavage Site Probability Heatmap

Stability Metrics

Detailed Analysis

Position	Amino Acid	Deuteration Level	Cathepsin B Score	Elastase Score
P1	Val	75%	0.82	0.24

Deuterated Peptide Linker Designer Documentation

Documentation for designing and analyzing deuterated peptide linkers with selective enzyme resistance.

Quick Start Guide

Input Sequence

Enter your peptide sequence using standard amino acid notation (e.g., Val-Cit)

Set Deuteration Parameters

Adjust deuteration levels using the slider (0-100%)

Analyze Results

Review enzyme cleavage predictions and stability metrics

Technical Details

Enzyme Specificity

Enzyme	Cleavage Pattern	Optimal pH
Cathepsin B	P1-P1' preference	5.0-6.5
Neutrophil Elastase	Small hydrophobic residues	7.0-8.5

Deuteration Effects

Increased metabolic stability
Modified enzyme recognition
Altered hydrogen bonding patterns
Impact on secondary structure

Algorithm Parameters


                                Scoring weights:
- Enzyme specificity: 0.4
- Deuteration level: 0.3
- Stability score: 0.3

Threshold values:
- Min. deuteration: 20%
- Max. deuteration: 95%
- Optimal range: 40-80%

Troubleshooting

Invalid Sequence Format

Ensure amino acids are separated by hyphens and use standard three-letter codes

Optimization Failures

Check if deuteration levels are within acceptable ranges

Performance Issues

Limit sequence length to 20 residues for optimal performance

API Reference

POST /api/analyze

Analyze peptide sequence


                            {
                                "sequence": "string",
                                "deuteration_level": number,
                                "enzymes": string[]
                            }

GET /api/results/{id}

Retrieve analysis results


                            {
                                "id": "string",
                                "status": "string",
                                "results": object
                            }